Western Blot: 12-Lipoxygenase Antibody [NBP2-29941] - Western blot analysis of ALOX12 Antibody (C-term) (NBP2-29941) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.
Immunocytochemistry/Immunofluorescence: 12-Lipoxygenase Antibody [NBP2-29941] - Immunofluorescent analysis of A549 cells, using ALOX12 Antibody (C-term) (NBP2-29941). NBP2-29941 was diluted at 1100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Immunohistochemistry-Paraffin: 12-Lipoxygenase Antibody [NBP2-29941] - Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry: 12-Lipoxygenase Antibody [NBP2-29941] - Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.