ALOX12

Gene Symbol ALOX12
Entrez Gene 239
Alt Symbol 12-LOX, 12S-LOX, LOG12
Species Human
Gene Type protein-coding
Description arachidonate 12-lipoxygenase
Other Description 12(S)-lipoxygenase|12S-lipoxygenase|arachidonate 12-lipoxygenase, 12S-type|lipoxin synthase 12-LO|platelet 12-LOX|platelet-type 12-lipoxygenase|platelet-type lipoxygenase 12
Swissprots P18054 Q9UQM4 Q6ISF8 O95569
Accessions AAA51587 AAS00094 BAA02162 EAW90281 P18054 AF143883 AAD32700 BC069557 AAH69557 M35418 AAA60056 M58704 AAA59523 M62982 AAA51533 S68587 AAD14020 S68588 AAD14021 XM_011523780 XP_011522082 NM_000697 NP_000688
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. {ECO:0000269|PubMed:16638750, ECO:0000269|PubMed:22237009, ECO:0000269|PubMed:23578768, ECO:0000269|PubMed:82
Subcellular Location Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF.
Tissue Specificity Expressed in vascular smooth muscle cells. {ECO:0000269|PubMed:23578768}.
Top Pathways Inflammatory mediator regulation of TRP channels, Arachidonic acid metabolism

12-LO (C-5) - sc-365194 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
12-LO (C-5): sc-365194. Western blot analysis of 12-LO expression in non-transfected 293T: sc-117752 (A), mouse 12-LO transfected 293T: sc-178202 (B) whole cell lysates and human platelet extract (C).

12-LO (H-100) - sc-32939 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
12-LO (H-100): sc-32939. Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue showing cytoplasmic staining of smooth muscle cells.

12-LO (L-20) - sc-27357 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
12-LO (L-20): sc-27357. Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.

Platelet-type 12-LO (C-20) - sc-27359 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-12 Lipoxygenase antibody - ab167372 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-12 Lipoxygenase antibody (ab167372) at 1 µg/mlLane 1 : 12 Lipoxygenase transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-12 Lipoxygenase antibody - ab84959 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-12 Lipoxygenase antibody (ab84959) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-12 Lipoxygenase antibody [ EPR11380(B)] - ab168384 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11380(B)
All lanes : Anti-12 Lipoxygenase antibody [ EPR11380(B)] (ab168384) at 1/1000 dilutionLane 1 : A431 lysateLane 2 : Human spleen lysateLane 3 : Human fetal liver lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-ALOX12 / 12 Lipoxygenase Antibody (aa578-627) - LS-C81851 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-ALOX12 / 12 Lipoxygenase Antibody - LS-C306215 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-ALOX12 / 12 Lipoxygenase Antibody - LS-C309424 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ALOX12 / 12 Lipoxygenase Antibody (clone 2D10) - LS-C196646 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D10
Detection limit for recombinant GST tagged ALOX12 is approximately 0.3 ng/ml as a capture antibody.

Anti-ALOX12 / 12 Lipoxygenase Antibody (aa352-656) - LS-C314862 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant ALOX12/12 Lipoxygenase/12-LOX.

Anti-ALOX12 / 12 Lipoxygenase Antibody (clone 5H2) - LS-C340386 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5H2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX12.

Anti-ALOX12 / 12 Lipoxygenase Antibody (clone 1C3) - LS-C340376 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1C3
IHC of paraffin-embedded Human pancreas tissue using anti-ALOX12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)(1:150).

Anti-ALOX12 / 12 Lipoxygenase Antibody (aa618-650) IHC-plus™ - LS-B10714 from LifeSpan Bioscience

Prices $395.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-ALOX12/12 Lipoxygenase/12-LOX antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10714 dilution 1:50.

12-Lipoxygenase Antibody - NBP2-29941 from Novus Biologicals

Prices $329.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western Blot: 12-Lipoxygenase Antibody [NBP2-29941] - Western blot analysis of ALOX12 Antibody (C-term) (NBP2-29941) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.

12-Lipoxygenase Antibody - NBP1-56327 from Novus Biologicals

Prices $329.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Western Blot: 12-Lipoxygenase Antibody [NBP1-56327] - Human Brain lysate, concentration 0.2-1 ug/ml.

12-Lipoxygenase Antibody - NBP1-90338 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: 12-Lipoxygenase Antibody [NBP1-90338] - Analysis in human cell line HELA.

12-Lipoxygenase Antibody - 27860002 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ELISA: 12 Lipoxygenase Antibody [27860002]

12-Lipoxygenase Antibody - H00000239-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: 12-Lipoxygenase Antibody [H00000239-B01P] - Analysis of ALOX12 expression in transfected 293T cell line by ALOX12 polyclonal antibody. Lane 1: ALOX12 transfected lysate(72.93 KDa). Lane 2: Non-transfected lysate.

12-Lipoxygenase Antibody - H00000239-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: 12 Lipoxygenase Antibody [H00000239-D01P] - Western blot analysis of ALOX12 expression in transfected 293T cell line by ALOX12 rabbit polyclonal antibody. Lane 1: ALOX12 transfected lysate. Lane 2: Non-transfected lysate.

12-Lipoxygenase Antibody (2D10) - H00000239-M01 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D10
ELISA: 12-Lipoxygenase Antibody (2D10) [H00000239-M01] - Detection limit for recombinant GST tagged ALOX12 is approximately 0.3ng/ml as a capture antibody.

12-Lipoxygenase Antibody - H00000239-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: 12-Lipoxygenase Antibody [H00000239-A01] - ALOX12 polyclonal antibody (A01), Lot # 051214JC01 Western Blot analysis of ALOX12 expression in HepG2 ( Cat # L019V1 ).

ALOX12/12 Lipoxygenase Antibody - bs-3874R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit

Monoclonal Anti-ALOX12, (C-terminal) antibody produced in mouse - SAB1402113 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D10
<B>ELISA</B><BR/>Monoclonal Anti-ALOX12, antibody produced in mouse, clone 2D10, purified immunoglobulin: <B>Cat. No. SAB1402113</B>: Detection limit for recombinant GST tagged ALOX12 is approximately 0.3 ng/mL.
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