In a functional flow cytometry test, biotinylated Recombinant Human R-Spondin 4 (100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with human Lgr4/GPR48 (dark orange histogram). Binding is completely blocked (light orange histogram) by 2.5 μg/mL of Mouse Anti-Human Lgr4/GPR48 Monoclonal Antibody (Catalog # MAB7750). Mouse IgG2B Isotype Control (Catalog # MAB004) at 2.5 μg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).
Lgr4/GPR48 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Mouse Anti-Human Lgr4/GPR48 Monoclonal Antibody (Catalog # MAB7750) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
HEK293 human embryonic kidney cell line transfected with human Lgr4/GPR48 and eGFP was stained with and either (A) Mouse Anti-Human Lgr4/GPR48 Monoclonal Antibody (Catalog # MAB7750) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.