Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL Rat Anti-Human RSK4 Monoclonal Antibody (Catalog # MAB3916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for RSK4 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows Recombinant Human Active RSK1 (Catalog # 992-KS), recombinant human RSK2, recombinant human RSK3, and recombinant human RSK4 (2 ng/lane). PVDF membrane was probed with 2 µg/mL Rat Anti-Human RSK4 Monoclonal Antibody (Catalog # MAB3916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for RSK4 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.