CDNF was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 10 µg/mL Goat Anti-Human CDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5097) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.