Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MDA‑MB‑468 human breast cancer cell line, C2C12 mouse myoblast cell line, C6 rat glioma cell line, and SH‑SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat DGK‑ gamma Monoclonal Antibody (Catalog # MAB6389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK‑ gamma at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.