DGKG

Gene Symbol	DGKG
Entrez Gene	1608
Alt Symbol	DAGK3, DGK-GAMMA
Species	Human
Gene Type	protein-coding
Description	diacylglycerol kinase, gamma 90kDa
Other Description	DAG kinase gamma\|diacylglycerol kinase gamma\|diacylglyerol kinase gamma\|diglyceride kinase gamma
Swissprots	Q2M1H4 P49619 Q5FWG1 B2RAH4
Accessions	AAC04686 P49619 AB209033 BAD92270 AK304440 BAH14187 AK309682 AK314192 BAG36871 AY737485 AAW63408 BC089411 AAH89411 BC112363 AAI12364 BM669549 D26135 BAA05132 NM_001080744 NP_001074213 NM_001080745 NP_001074214 NM_001346 NP_001337
Function	Reverses the normal flow of glycerolipid biosynthesis by phosphorylating diacylglycerol back to phosphatidic acid.
Subcellular Location	Cytoplasm. Membrane. Note=Can be loosely bound to the membranes.
Tissue Specificity	Predominantly expressed in retina and in a much lesser extent in the brain. Other tissues contain extremely low levels of DGK-gamma.
Top Pathways	Choline metabolism in cancer, Glycerolipid metabolism, Glycerophospholipid metabolism, Phosphatidylinositol signaling system

DGK-γ (H-56) - sc-98621 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

DGK-γ (N-20) - sc-49184 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

DGK-γ (N-20): sc-49184. Western blot analysis of DGK-γ expression in HeLa whole cell lysate.

DGK-γ (Y-15) - sc-49185 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-DGKG antibody - ab151967 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DGKG antibody (ab151967) at 1/1000 dilution + H1299 lysate at 30 µg

Anti-DGKG antibody - ab89037 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-DGKG antibody (ab89037) at 1 µg/ml + Rat brain tissue lysate at 50 µg

Anti-DGKG antibody - ab37609 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DGKG antibody (ab37609) at 1/100 dilution + HeLa cell lysate

Anti-DGKG Antibody (aa451-698, FITC) - LS-C304001 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DGKG Antibody (aa451-698, Biotin) - LS-C301084 from LifeSpan Bioscience

Prices	$425.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DGKG Antibody (aa287-524, FITC) - LS-C304003 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DGKG Antibody (aa287-524, Biotin) - LS-C301086 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DGKG Antibody - LS-C307230 from LifeSpan Bioscience

Prices	$455.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-DGKG Antibody - LS-C311088 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DGKG Antibody (aa451-698) - LS-C293679 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DGKG Antibody (aa287-524) - LS-C293682 from LifeSpan Bioscience

Prices	$425.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

DGK-gamma Antibody (613531) - MAB6389 from Novus Biologicals

Prices	$99.00, $249.00
Sizes	25 µg, 100 µg
Host	Mouse
Clonality	Monoclonal
Clone	613531

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MDA‑MB‑468 human breast cancer cell line, C2C12 mouse myoblast cell line, C6 rat glioma cell line, and SH‑SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat DGK‑ gamma Monoclonal Antibody (Catalog # MAB6389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK‑ gamma at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DGK-gamma Antibody - NBP1-33055 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: DGK-gamma Antibody [NBP1-33055] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE; antibody diluted at 1:1000.

DGK-gamma Antibody - NBP1-85316 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: DGKG Antibody [NBP1-85316] Staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.

DGK-gamma Antibody - H00001608-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

DGK-gamma Antibody - H00001608-B01 from Novus Biologicals

Prices	$349.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: DGKG Antibody [H00001608-B01] - DGKG MaxPab polyclonal antibody. Western Blot analysis of DGKG expression in rat brain.

DGK-gamma Antibody - H00001608-D01 from Novus Biologicals

Prices	$399.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DGKG antibody produced in mouse - SAB1405696 from Sigma-Aldrich

Prices	$347.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-DGKG: <B>Cat. No. SAB1405696</B>: Antibody concentration: 1-5 μg/mL. Specific band of ~89 kDa using rat brain lysate.

Anti-DGKG (446-460) antibody produced in rabbit - D5195 from Sigma-Aldrich

Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human DGKG was separated on SDS-PAGE, blotted with Anti-DGKG (446-460) (<B>Cat. No. D5195</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500

Anti-DGKG (N-term) antibody produced in rabbit - SAB1300470 from Sigma-Aldrich

Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-DGKG (N-term) Antibody: <B>Cat. No. SAB1300470</B>: DGKG in human hepatocarcinoma tissue with Anti-DGKG (N-term) Antibody at 40 μg/mL.

Anti-DGKG (17-30) antibody produced in rabbit - D5070 from Sigma-Aldrich

Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunofluorescence</B><BR/>Anti-DGKG (17-30): <B>Cat. No. D5070</B>: Immunofluorescence of HUVEC cells using DGKG (17-30), Cat. No. D5070 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-DGKG (C-term) antibody produced in rabbit - SAB1300471 from Sigma-Aldrich

Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-DGKG (C-term) Antibody: <B>Cat. No. SAB1300471</B>: DGKG in HeLa cell line lysates with Anti-DGKG (C-term) Antibody at 4.0 μg/mL.

Search more