Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat DYRK3 Monoclonal Antibody (Catalog # MAB5409) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for DYRK3 at approximately 70 kDa and 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.