Western blot shows recombinant human MafF, MafG, and MafK (5 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.