Western blot shows lysates of JAR human choriocarcinoma cell line, beta TC‑6 mouse beta cell insulinoma cell line, and NRK‑49F rat fibroblasts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat NEDP1/SENP8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7760) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NEDP1/SENP8 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.