Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4958) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Human Plexin B3 (Catalog # 4958-PC) (1 ng) was included. A specific band for Plexin B3 was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
A172 human glioblastoma cell line was stained with Sheep Anti-Human Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4958, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).