Western Blot: PALB2 Antibody [21340002] - Western Blot was performed using affinity purified SEQer PALB2 antibody, aa(99-198) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer PALB2 antibody, aa(99-198) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
Immunohistochemistry: PALB2 Antibody [21340002] - Normal and malignant tissues exhibited strong nuclear membrane and additional cytoplasmic staining. Testicular tumors and prostate cancers showed in most cases moderate cytoplasmic positivity. Image and statement courtesy of the Human Protein Atlas (HPA).