Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line, CEM human T-lymphoblastoid cell line, Huh‑7 human hepatoma cell line, Neuro‑2A mouse neuroblastoma cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Nicalin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7686) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nicalin at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.