Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3-Kinase p55 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5734) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PI 3-Kinase p55 gamma at approximately 55 to 57 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.