Immunohistochemistry: CASC5 Antibody [NB100-2586] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-CASC5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Western Blot: CASC5 Antibody [NB100-2586] - Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Affinity purified rabbit anti-CASC5 antibody NB100-2586 lot 2 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. CASC5 was also immunoprecipitated by a previous lot 1 of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B).
Immunohistochemistry-Paraffin: CASC5 Antibody [NB100-2586] - FFPE section of human lung carcinoma. Affinity purified rabbit anti-CASC5 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: CASC5 Antibody [NB100-2586] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).