Western Blot: HA95/AKAP8L Antibody [NB100-61606] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-61606 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. AKAP8L/HA95 was also immunoprecipitated by rabbit anti-AKAP8L/HA95 antibody NB100-61605, which recognizes an upstream epitope.
Immunocytochemistry/Immunofluorescence: HA95/AKAP8L Antibody [NB100-61606] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-AKAP8L/HA95 used at a dilution of 1:100.
Immunohistochemistry: HA95/AKAP8L Antibody [NB100-61606] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-AKAP8L/HA95 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.