Western Blot: TIP120A Antibody [NBP1-49918] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TIP120A antibody NBP1-49918 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. TIP120A was also immunoprecipitated by rabbit anti-TIP120A antibody NBP1-49917, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunohistochemistry: TIP120A Antibody [NBP1-49918] - Sample: FFPE section of human ovarian carcinoma (left) and mouse hybridoma tumor (right). Antibody: Affinity purified rabbit anti-CAND1 used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB