Spi-B (D3C5E) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 14323
• Prices: $246.00
• Sizes: 100 µl (50 immunoprecipitations)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D3C5E
• Applications: IP ICC/IF FC
• Species Reactivities: Human
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala86 of human Spi-B protein.
• Description: Rabbit Monoclonal
• Gene: SPIB

Product images

Immunoprecipitation of Spi-B from Raji cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Spi-B (D3C5E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Spi-B (D4V9S) Rabbit mAb #14337.

Confocal immunofluorescent analysis of Raji (positive, left) and Jurkat (negative, right) cells using Spi-B (D3C5E) Rabbit mAb (green). Actin filaments were labeled with DyLight ® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) using Spi-B (DC35E) Rabbit mAb co-stained with BDCA-2, CD19, and CD3. BDCA-2+ plasmacytoid dendritic cells and CD19+ B cells are positive for Spi-B, whereas CD3+ T cells are negative. Anti-rabbit IgG (H+L), (F(ab')2 ) Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.

Flow cytometric analysis of Jurkat cells (blue) and Raji cells (green) using Spi-B (D3C5E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.