Western Blot: CCNL1 Antibody [NBP1-77747] - Shows detection of a band ~35 kDa corresponding to Cyclin L b(arrowhead) present in mouse brain whole cell lysate (800 nm channel - green). Marker proteins appear red (700 nm channel) and were used for molecular weight comparisons. Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:2,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye ™ 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
Immunohistochemistry-Paraffin: CCNL1 Antibody [NBP1-77747] - This antibody was used at a 10ug/mL in a variety of tissues including breast, kidney, liver, lung, skeletal muscle, pancreas, prostate and spleen. In some tissues elevated background staining was noted. In these instances further optimization of dilution is suggested. This image shows Cyclin L staining of human skeletal muscle.