Western Blot: SIN3B Antibody [NBP2-20367] - Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE diluted at 1:500
Immunocytochemistry/Immunofluorescence: SIN3B Antibody [NBP2-20367] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3B protein stained by SIN3B antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367] - Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using antibody at 1:100 dilution.
Immunoprecipitation: SIN3B Antibody [NBP2-20367] - Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 ug of SIN3B antibody [N1N2], N-term Western blot analysis was performed using SIN3B antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.