SPT16 (D7I2K) Rabbit mAb
| Name | SPT16 (D7I2K) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 12191 |
| Prices | $246.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D7I2K |
| Applications | WB ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey, Hamster, Xenopus, Zebrafish, Bovine, Dog, Guinea Pig, Horse |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu662 of human SPT16 protein. |
| Description | Rabbit Monoclonal |
| Gene | SUPT16H |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using SPT16 (D7I2K) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HCT 116 cells starved for 48 hr then serum stimulated with 20% FBS for 15 min and either 10 μl of SPT16 (D7I2K) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human c-Fos Promoter Primers #4663, SimpleChIP ® Human c-Fos Exon 3 Primers #12010, SimpleChIP ® Human EGR1 Promoter Primers #5549, and SimpleChIP ® Human EGR1 Intron 3 Primers #11953. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.