Western blot analysis of extracts from A549 cells, untreated (-) or treated with Roscovitine #9885 (20 μM, overnight; +), using Phospho-SQSTM1/p62 (Thr269/Ser272) Antibody (upper), SQSTM1/p62 (D5E2) Rabbit mAb #8025 (middle) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from chloroquine-treated HeLa cells (50 μM, overnight; +) and untreated (-) MCF7 cells, untreated (-) or treated with λ phosphatase and calf intestinal phosphatase (CIP) (+), using Phospho-SQSTM1/p62 (Thr269/Ser272) Antibody (upper) and SQSTM1/p62 (D5E2) Rabbit mAb #8025 (lower).
Immunoprecipitation of Phospho-SQSTM1/p62 (Thr269/Ser272) from ACHN cells using Normal Rabbit IgG #2729 (lane 2) or Phospho-SQSTM1/p62 (Thr262/Ser272) Antibody (lane 3). Lane 1 is 10% input. Western blot was performed using Phospho-SQSTM1/p62 (Thr262/Ser272) Antibody. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb