Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (20 ng/ml, overnight; -) followed by transfection with poly(deoxyadenylic-deoxythymidylic) acid (poly(dA:dT); 2 μg/ml, 7 hr; +) using Phospho-SQSTM1/p62 (Ser403) Antibody (upper) or total SQSTM1/p62 (D5E2) Rabbit mAb #8025 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing GFP-tagged human SQSTM1/p62 (hSQSTM1/p62-GFP; +) and human GST-tagged IKK-ε (hIKK-ε-GST; +) as indicated using Phospho-SQSTM1/p62 (Ser403) Antibody (upper) or SQSTM1/p62 (D5E2) Rabbit mAb #8025 (lower).