Western Blot: Ext2 Antibody [NBP2-29948] - Western blot analysis of lysate from T47D cell line,using EXT2 Antibody (Center)(NBP2-29948).AP9401c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
Immunocytochemistry/Immunofluorescence: Ext2 Antibody [NBP2-29948] - Confocal immunofluorescent analysis of EXT2 Antibody (Center)(NBP2-29948) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemistry-Paraffin: Ext2 Antibody [NBP2-29948] - Formalin-fixed and paraffin-embedded human brain tissue reacted with EXT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry: Ext2 Antibody [NBP2-29948] - Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.