Western Blot: UBE2M/Ubc12 Antibody (2D9) [NBP2-01377] - Analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2M monoclonal antibody.
Immunocytochemistry/Immunofluorescence: UBE2M/Ubc12 Antibody (2D9) [NBP2-01377] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M.
Western Blot: UBE2M/Ubc12 Antibody (2D9) [NBP2-01377] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2M (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2M.