UBE2M

Gene Symbol UBE2M
Entrez Gene 9040
Alt Symbol UBC-RS2, UBC12, hUbc12
Species Human
Gene Type protein-coding
Description ubiquitin-conjugating enzyme E2M
Other Description NEDD8 carrier protein|NEDD8 protein ligase|NEDD8-conjugating enzyme Ubc12|ubiquitin carrier protein M|ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)|ubiquitin-protein ligase M|yeast UBC12 homolog
Swissprots P61081
Accessions CDM22105 EAW72612 EAW72613 P61081 AB012191 BAA33145 AF075599 AAC26141 AL569047 BC058924 AAH58924 BM559596 BM798750 BT006754 AAP35400 BU508830 BX342697 NM_003969 NP_003960
Function Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. {ECO:0000269|PubMed:10207026, ECO:0000269|PubMed:15361859}.
Top Pathways Ubiquitin mediated proteolysis

Ubc12 (D13D7) Rabbit mAb - 5641 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D13D7
Western blot analysis of extracts from various cell lines using Ubc12 (D13D7) Rabbit mAb.

Ubc12 Antibody - 4913 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Ubc12 Antibody.

UBC12 (C-20) - sc-79184 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
UBC12 (C-20): sc-79184. Western blot analysis of UBC12 expression in A549 (A), U-937 (B), MCF7 (C), NIH/3T3 (D), RAW 264.7 (E) and Jurkat (F) whole cell lysates.

UBC12 (Y-20) - sc-79187 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

UBC12 (D-4) - sc-390064 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
UBC12 (D-4): sc-390064. Western blot analysis of UBC12 expression in Caki-1 (A), Ramos (B), 293T (C), Raji (D) and Hep G2 (E) whole cell lysates.

UBC12 (H-42) - sc-366017 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

UBC12 (L-34) - sc-100608 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
UBC12 (L-34): sc-100608. Western blot analysis of UBC12 expression in non-transfected: sc-117752 (A) and human UBC12 transfected: sc-116642 (B) 293T whole cell lysates.

Anti-Ube2M antibody - ab56383 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-UBE2M antibody [EPR5333] - ab109507 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5333
Overlay histogram showing Ramos cells stained with ab109507 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109507, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-UBE2M antibody [EPR5334] - ab124946 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5334
All lanes : Anti-UBE2M antibody [EPR5334] (ab124946) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Ramos cell lysateLysates/proteins at 10 µg per lane.Secondarystandard HRP labelled goat anti-rabbit at 1/2000 dilution

Anti-UBE2M antibody - C-terminal - ab200453 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-UBE2M antibody - C-terminal (ab200453) at 1/1000 dilution + mouse brain total cell extract

Anti-UBE2M / UBC12 Antibody (aa169-180) IHC-plus™ - LS-B432 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-UBE2M antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B432 concentration 5 ug/ml.

Anti-UBE2M / UBC12 Antibody (C-Terminus, clone EPR5333) - LS-C138461 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5333
IHC of paraffin-embedded human lung carcinoma tissue using anti-UBC12 antibody.

Anti-UBE2M / UBC12 Antibody (clone 2D9) - LS-C340598 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2D9
Anti-UBE2M mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M.

Anti-UBE2M / UBC12 Antibody (N-Terminus) - LS-C80539 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-UBE2M / UBC12 Antibody (Internal, clone EPR5334) - LS-C138655 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5334
Western blot analysis on (A) 293T and (B) Ramos cell lysates using anti-UBC12 antibody.

UBE2M/Ubc12 Antibody (EPR5333) - NBP1-95709 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5333
Western Blot: UBE2M Antibody (EPR5333) [NBP1-95709] - (A) 293T and (B) Ramos cell lysates.

UBE2M/Ubc12 Antibody - NBP1-55035 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035] - Human 721_B, Antibody Dilution: 1.0 ug/ml UBE2M is supported by BioGPS gene expression data to be expressed in 721_B.

UBE2M/Ubc12 Antibody (2D9) - NBP2-01377 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2D9
Western Blot: UBE2M/Ubc12 Antibody (2D9) [NBP2-01377] - Analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2M monoclonal antibody.

UBE2M/Ubc12 Antibody - A-655 from Novus Biologicals

Prices $159.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

UBE2M/Ubc12 Antibody (3C4) - H00009040-M01 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3C4
Western Blot: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - UBE2M expression in Jurkat ( Cat # L017V1 ).

UBE2M/Ubc12 Antibody - NBP1-31459 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UBE2M/Ubc12 Antibody [NBP1-31459] - Sample(30 ug whole cell lysate) A:293T 12% SDS PAGE, antibody diluted at 1:1000.

UBE2M/Ubc12 Antibody (1G9) - H00009040-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1G9

UBE2M Polyclonal Antibody - bs-8382R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Monoclonal Anti-UBE2M antibody produced in mouse - WH0009040M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3C4
<B>Immunoblotting</B><BR/>Monoclonal Anti-UBE2M, <B>Cat. No. WH0009040M1</B> used at the antibody concentration: 1 ~ 5 μg/mL. Specific band of ~20.9 kDa using Jurkat lysate.
Search more