Western blot analysis of extracts from 293T cells, untreated (-) or treated (+) with λ phosphatase and calf intestinal phosphatase (CIP), using Phospho-Src (Ser17) (D7F2Q) Rabbit mAb (upper) or Src (32G6) Rabbit mAb #2123 (lower).
Immunoprecipitation of phospho-Src (Ser17) from 293T cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-Src (Ser17) (D7F2Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Src (Ser17) (D7F2Q) Rabbit mAb. A light-chain specific antibody was used as a secondary antibody.