CCL3/MIP‑1 alpha was detected in perfusion fixed frozen sections of rat kidney using Mouse Anti-Rat CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB66251) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.