Ccl3

Gene Symbol Ccl3
Entrez Gene 25542
Alt Symbol MIP-1a, Scya3
Species Rat
Gene Type protein-coding
Description chemokine (C-C motif) ligand 3
Other Description C-C motif chemokine 3|MIP-1-alpha|Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3)|macrophage inflammatory protein 1-alpha|small inducible cytokine A3|small-inducible cytokine A3
Swissprots A1EC87 P50229
Accessions EDM05492 P50229 EF121994 ABL63433 EF121995 ABL63434 U06435 AAA96498 U22414 AAA80608 NM_013025 NP_037157
Function Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
Subcellular Location Secreted.
Top Pathways Cytokine-cytokine receptor interaction, Toll-like receptor signaling pathway, Chemokine signaling pathway, Chagas disease (American trypanosomiasis), Rheumatoid arthritis

Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody - ab9781 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Indirect ELISA measuring Macrophage Inflammatory Protein 1 alpha / CCL3 levels using ab9781. Supernatants collected from rat cerebral cortical cells after various treatments were coated onto 96-well flat bottom plates and incubated overnight at 4°C. The plates were blocked with 1% BSA at 37°C for 45 min before incubation with primary antibody (1/1000) in bicarbonate buffer overnight at 4°C. An HRP-conjugated secondary IgG (1/1000) was added and incubated for 45 min at 37°C, in the dark. The reaction was developed by adding o-phenylene diamine H2O2 for 20 min. Optical density was measured at 450 nm.

Anti-CCL3 / MIP-1-Alpha Antibody - LS-C25333 from LifeSpan Bioscience

Prices $560.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CCL3 / MIP-1-Alpha Antibody (AP) - LS-C87085 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-CCL3 / MIP-1-Alpha Antibody - LS-C104774 from LifeSpan Bioscience

Prices $285.00, $325.00
Sizes 50 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Sandwich ELISA of CCL3 antibody LS-C104774

Anti-CCL3 / MIP-1-Alpha Antibody (Biotin) - LS-C104705 from LifeSpan Bioscience

Prices $285.00, $325.00
Sizes 25 µg, 50 µg
Host Rabbit
Clonality Polyclonal
Sandwich ELISA of CCL3 antibody LS-C104705

Anti-CCL3 / MIP-1-Alpha Antibody - LS-C292481 from LifeSpan Bioscience

Prices $365.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal

Anti-CCL3 / MIP-1-Alpha Antibody (Biotin) - LS-C298922 from LifeSpan Bioscience

Prices $425.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal

Anti-CCL3 / MIP-1-Alpha Antibody (FITC) - LS-C303460 from LifeSpan Bioscience

Prices $445.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal

Anti-CCL3 / MIP-1-Alpha Antibody (aa24-92) - LS-C313417 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
WB of CCL3 / MIP-1-Alpha antibody. All lanes: Anti-CCL3 at 0.5ug/ml. WB: Recombinant Rat CCL3 Protein 0.5ng. Predicted bind size: 33KD. Observed bind size: 33KD.

Anti-CCL3 / MIP-1-Alpha Antibody (aa24-92) - LS-C314383 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant CCL3 / MIP-1-Alpha.

CCL3/MIP-1 alpha Antibody (769303) - MAB66251 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 769303
CCL3/MIP‑1 alpha was detected in perfusion fixed frozen sections of rat kidney using Mouse Anti-Rat CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB66251) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

CCL3/MIP-1 alpha Antibody (755012) - MAB6625 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 755012
Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB6625) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL3/MIP‑1 alpha at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CCL3/MIP-1 alpha Antibody - AF1061 from Novus Biologicals

Prices $99.00, $369.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Recombinant Cotton Rat CCL3/MIP‑1 alpha (Catalog # 1061‑RM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP‑1 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL3/MIP‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1061). The ND50 is typically 0.1-0.4 µg/mL.

CCL3/MIP-1 alpha Antibody - AF6625 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal
Western Blot CCL3/MIP-1 alpha Antibody

CCL3/MIP-1 alpha Antibody [Biotin] - BAF1061 from Novus Biologicals

Prices $420.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti -CCL3 (Macrophage Inflammatory Protein 1 alpha, MIP-1 alpha, LD78a) - 145408 from USBiological Life Science

Host Mouse
Clonality Monoclonal
Clone 769324

Anti -MIP-1a (Macrophage Inflammatory Protein-1 a) - M1202-05 from USBiological Life Science

Host Rabbit
Clonality Polyclonal

MIP1 alpha / CCL3 antibody - PP038B1 from Acris Antibodies

Prices $235.00
Sizes 25 µg
Host Rabbit
Clonality Polyclonal

MIP1 alpha / CCL3 antibody - PP038B2 from Acris Antibodies

Prices $310.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

MIP1 alpha / CCL3 antibody - PP038P1 from Acris Antibodies

Prices $235.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

MIP1 alpha / CCL3 antibody - PP038P2 from Acris Antibodies

Prices $310.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

MIP1 alpha / CCL3 antibody - PAB8837 from Acris Antibodies

Prices $500.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of Ccl3 with RAW 264.7 cells treated with LPS.

Rabbit Anti-MIP-1-alpha Antibody - 168-10867 from RayBiotech

Host Rabbit
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