Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 LS180 cells treated with calcitriol (10nM, 3 hours) and either 10 μl of SRC-1 (128E7)) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human c-Fos Upstream Primers #25661 and SimpleChIP ® Human Nanog Promoter Primers #95064. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.