Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Lysosomal Pro‑X Carboxypeptidase/PRCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7164) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysosomal Pro‑X Carboxypeptidase/PRCP at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.