Western blot analysis of cell extracts from MCF-7 cells, untreated or stimulated with heregulin, using Phospho-SRC-3 (Thr24) Antibody (upper and middle) or SRC-3 (5E11) Rabbit mAb #2126 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing.
Confocal immunofluorescent analysis of HeLa cells, either untreated (left) or λ-phosphatase treated (right), using Phospho-SRC-3 (Thr24) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).