NCOA3

Gene Symbol NCOA3
Entrez Gene 8202
Alt Symbol ACTR, AIB-1, AIB1, CAGH16, CTG26, KAT13B, RAC3, SRC-3, SRC3, TNRC14, TNRC16, TRAM-1, bHLHe42, pCIP
Species Human
Gene Type protein-coding
Description nuclear receptor coactivator 3
Other Description CBP-interacting protein|amplified in breast cancer 1 protein|class E basic helix-loop-helix protein 42|receptor-associated coactivator 3|steroid receptor coactivator protein 3|thyroid hormone receptor activator molecule 1
Swissprots Q9BR49 Q9UPG7 Q0VF45 Q9Y6Q9 Q5JYD9 Q9UPG4 A4LAZ5 Q5JYE0 Q9UPC9
Accessions ABO43042 EAW75696 EAW75697 EAW75698 EAW75699 EAW75700 EAW75701 EAW75702 Q9Y6Q9 AB209863 BAD93100 AF010227 AAC51663 AF012108 AAC51677 AF016031 AAC51849 AF036892 AAB92368 AK289683 BAF82372 AK296810 BAG59383 AK302588 BAG63841 AK302595 BAG63847 AK308346 BC092516 AAH92516 BC119001 AAI19002 BC122547 AAI22548 BM692321 BU588121 CA450268 DB091786 NM_001174087 NP_001167558 NM_001174088 NP_001167559 NM_006534 NP_006525 NM_181659 NP_858045
Function Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
Subcellular Location Cytoplasm. Nucleus. Note=Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
Tissue Specificity Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.

Phospho-SRC-3 (Thr24) Antibody - 2979 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of cell extracts from MCF-7 cells, untreated or stimulated with heregulin, using Phospho-SRC-3 (Thr24) Antibody (upper and middle) or SRC-3 (5E11) Rabbit mAb #2126 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing.

SRC-3 (5E11) Rabbit mAb - 2126 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 5E11
Western blot analysis of cell lysates from A549, HeLa and Jurkat cells using SRC-3 (5E11) Rabbit mAb.

SRC-3 (11B1) Mouse mAb - 2115 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 11B1
Western blot analysis of cell extracts from various cell lines, using SRC-3 (11B1) Mouse mAb.

SRC-3 (D1F11) Rabbit mAb - 5765 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1F11
Western blot analysis of extracts from various cell lines using SRC-3 (D1F11) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

NCoA-3 (AX 15) - sc-56856 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCoA-3 (AX 15): sc-56856. Western blot analysis of NCoA-3 expression in K-562 whole cell lysate.

NCoA-3 (1A8) - sc-80992 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCoA-3 (1A8): sc-80992. Western blot analysis of NCoA-3 expression in MCF7 whole cell lysate.

NCoA-3 (AC3) - sc-56855 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCoA-3 (AC3): sc-56855. Western blot analysis of NCoA-3 expression in K-562 whole cell lysate.

NCoA-3 (AX15.3) - sc-33671 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCoA-3 (AX15.3): sc-33671. Western blot analysis of NCoA-3 expression in HeLa whole cell lysate.

NCoA-3 (C-20) - sc-7216 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NCoA-3 (C-20): sc-7216. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining.

NCoA-3 (E-11) - sc-166751 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCoA-3 (E-11): sc-166751. Western blot analysis of NCoA-3 expression in HeLa nuclear extract (A) and HeLa whole cell lysate (B).

NCoA-3 (F-2) - sc-5305 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ChIP analysis of <i>in vivo</i> binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included SRC-1 (M-341): sc-8995, SRC-1 (C-20): sc-6096 and SRC-1 (M-20): sc-6098 (B), Grip-1 (M-434): sc-8996 (C), NCoA-3 (F-2): sc-5305, NCoA-3 (M-397): sc-9119, NCoA-3 (N-17): sc-7217 and NCoA-3 (C-20): sc-7216 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold <i>et al</i>., Neuron 2003, 40: 1119-1131.

NCoA-3 (H-80) - sc-25742 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NCoA-3 (H-80): sc-25742. Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

NCoA-3 (H-270) - sc-13066 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NCoA-3 (H-270): sc-13066. Western blot analysis of NCoA-3 expression in MIA PaCa-2 whole cell lysate.

NCoA-3 (K-15) - sc-14607 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NCoA-3 (K-15): sc-14607. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.

NCoA-3 (M-397) - sc-9119 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NCoA-3 (M-397): sc-9119. Western blot analysis of His-tagged mouse recombinant NCoA-3.

NCoA-3 (N-16) - sc-14605 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NCoA-3 (N-16): sc-14605. Western blot analysis of NCoA-3 expression in MIA PaCa-2 whole cell lysate.

NCoA-3 (R-19) - sc-32348 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NCoA-3 (R-19): sc-32348. Western blot analysis of NCoA-3 expression in Jurkat whole cell lysate.

NCoA-3 (N-17) - sc-7217 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ChIP analysis of <i>in vivo</i> binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included SRC-1 (M-341): sc-8995, SRC-1 (C-20): sc-6096 and SRC-1 (M-20): sc-6098 (B), Grip-1 (M-434): sc-8996 (C), NCoA-3 (F-2): sc-5305, NCoA-3 (M-397): sc-9119, NCoA-3 (N-17): sc-7217 and NCoA-3 (C-20): sc-7216 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold <i>et al</i>., Neuron 2003, 40: 1119-1131.

NCoA-3 (6D102) - sc-71657 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCoA-3 (6D102): sc-71657. Western blot analysis of NCoA-3 expression in Jurkat (A) and K-562 (B) whole cell lysates.

NCoA-3 (4i301) - sc-71654 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

NCoA-3 (0.T.198) - sc-56854 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCoA-3 (0.T.198): sc-56854. Western blot analysis of NCoA-3 expression in Jurkat (A) and K-562 (B) whole cell lysates.

Anti-NCOA3 antibody [5H3] - ab119913 from Abcam

Host Mouse
Clonality Monoclonal
Clone 5H3
ELISA image showing use of ab119913.

Anti-SRC3 antibody - ab10310 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Detection of SCR3 by Immunoprecipitation. Samples: Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 µg/ml extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3.

Anti-SRC3 antibody - ab85452 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-SRC3 antibody - ab10313 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Detection of SRC3 by immunoprecipitation using ab10313 at 20µg/10mg extract. Samples: 10mg nuclear extract from HeLa cells. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3.
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