Western blot shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line and human ovary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Prostaglandin I2 Synthase Monoclonal Antibody (Catalog # MAB7788) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Prostaglandin I2 Synthase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line and human ovarian cancer, loaded at 0.5 mg/mL. A specific band was detected for Prostaglandin I2 Synthase at approximately 55 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Prostaglandin I2 Synthase Monoclonal Antibody (Catalog # MAB7788). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.