Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, beta TC‑6 mouse beta cell insulinoma cell line, OVCAR‑3 human ovarian carcinoma cell line, and SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human APPL Monoclonal Antibody (Catalog # MAB7075) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for APPL at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.