Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Tenascin X Monoclonal Antibody (Catalog # MAB6999) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tenascin X at approximately 470 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Simple Western lane view shows lysates of human heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Tenascin X at approximately 376 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Tenascin X Monoclonal Antibody (Catalog # MAB6999). This experiment was conducted under reducing conditions and using the66-440 kDa separation system.