Western blot shows lysates of NCI‑H460 human large cell lung carcinoma cell line, human lung tissue, and human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Hematopoietic Prostaglandin D Synthase/HPGDS Monoclonal Antibody (Catalog # MAB6487) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Hematopoietic Prostaglandin D Synthase/HPGDS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hematopoietic Prostaglandin D Synthase/HPGDS was detected in immersion fixed HEL 92.1.7 human erythroleukemic cell line using Mouse Anti-Human Hematopoietic Prostaglandin D Synthase/HPGDS Monoclonal Antibody (Catalog # MAB6487) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.