Western blot shows nuclear extracts of HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PIAS3 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5120) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PIAS3 at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.