Recombinant Cotton Rat TNF‑ alpha (Catalog # 1011-CR) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Cotton Rat TNF‑ alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1011). The ND50 is typically 0.01-0.05 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).