Tnf

Gene Symbol Tnf
Entrez Gene 24835
Alt Symbol RATTNF, TNF-alpha, Tnfa
Species Rat
Gene Type protein-coding
Description tumor necrosis factor
Other Description cachectin|tumor necrosis factor (TNF superfamily, member 2)|tumor necrosis factor alpha|tumor necrosis factor ligand superfamily member 2
Swissprots Q6EE11 Q9JI27 P16599 Q9JI26
Accessions AAA16275 AAA42255 AAK53568 AAK53569 AAK53570 AAK53571 AAK53572 AAK53573 AAR91624 AAR91625 AAR91626 BAA00367 CAE84003 EDL83547 EDL83548 P16599 AF269159 AAF82567 AF269160 AAF82568 AJ002278 CAA05290 BC107671 AAI07672 X66539 CAA47146 NM_012675 NP_036807
Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Subcellular Location C-domain 2: Secreted {ECO:0000250}.
Top Pathways Rheumatoid arthritis, Inflammatory bowel disease (IBD), Sphingolipid signaling pathway, Non-alcoholic fatty liver disease (NAFLD), Cytokine-cytokine receptor interaction

Anti-TNF alpha antibody - ab9755 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab9755 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9755, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab9755, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

TNF-alpha Antibody [Unconjugated] - AF-510-NA from Novus Biologicals

Prices $99.00, $365.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Recombinant Rat TNF‑ alpha (Catalog # 510-RT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Rat TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-510-NA). The ND50 is typically 0.15‑0.75 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNF-alpha Antibody (45418) - MAB510 from Novus Biologicals

Prices $99.00, $269.00, $699.00
Sizes 25 µg, 100 µg, 500 µg
Host Mouse
Clonality Monoclonal
Clone 45418
Recombinant Rat TNF‑ alpha (Catalog # 510-RT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rat TNF‑ alpha (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF‑ alpha Monoclonal Antibody (Catalog # MAB510). The ND50 is typically 10-40 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNF-alpha Antibody [Unconjugated] - AF1011 from Novus Biologicals

Prices $99.00, $329.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Recombinant Cotton Rat TNF‑ alpha (Catalog # 1011-CR) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Cotton Rat TNF‑ alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1011). The ND50 is typically 0.01-0.05 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNF-alpha Antibody [Biotin] - BAF510 from Novus Biologicals

Prices $390.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

TNF-alpha Antibody [Biotin] - BAF1011 from Novus Biologicals

Prices $390.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in mouse - T3198 from Sigma-Aldrich

Prices $463.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone 45418.111

TNF antibody - 70R-7804 from Fitzgerald

Prices $675.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

TNF alpha antibody - 70R-11584 from Fitzgerald

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

TNFa Antibody - abx102789 from Abbexa

Host Rabbit
Clonality Polyclonal

Tumor necrosis factor (TNF-alpha) antibody - PP039B1 from Acris Antibodies

Prices $235.00
Sizes 25 µg
Host Rabbit
Clonality Polyclonal

Tumor necrosis factor (TNF-alpha) antibody - PP039B2 from Acris Antibodies

Prices $310.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Tumor necrosis factor (TNF-alpha) antibody - PP039P1 from Acris Antibodies

Prices $235.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Tumor necrosis factor (TNF-alpha) antibody - PP039P2 from Acris Antibodies

Prices $310.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Tumor necrosis factor (TNF-alpha) antibody - PAB9571 from Acris Antibodies

Prices $430.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis with recombinant rat Tnf. Lane 1 : 10 ng. Lane 2 : 50 ng. Lane 3 : 250 ng.

Rabbit Anti-TNF-alpha Antibody - 168-10041 from RayBiotech

Host Rabbit

Rat Anti-Mouse TNF-alpha - DS-MB-02427 from RayBiotech

Host Rat

Rat Anti-Mouse TNF-alpha - DS-MB-02430 from RayBiotech

Host Rat

Rabbit Anti-Rat TNF-alpha - DS-PB-01977 from RayBiotech

Host Rabbit

Anti-Tumor Necrosis Factor Alpha (TNFa) - 20-2321 from ARP American Research Products

Prices $209.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

TNF-a Antibody - XP-5286 from ProSci

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
To detect Rat TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat TNF-alpha (XP-5286Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-alpha.

TNF-a Antibody (biotin) - XP-5286Bt from ProSci

Prices $315.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
To detect Rat TNF-alpha by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat TNF-alpha.

TNF-alpha / TNFA Antibody, Rabbit PAb, Antigen Affinity Purified - 80045-RP02-50 from Sino Biological

Prices $99.00, $99.00, $99.00
Sizes 50 µg, 100 µg, 200 µg
Host Rabbit
Clonality Polyclonal

Rabbit Anti-Rat Tnf Alpha Polyclonal antibody, Biotin-Conjugated - CPBT-65309RR from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Anti-TNF alpha polyclonal antibody - CPBT-65310RR from Creative Diagnostics

Host Rabbit
Clonality Polyclonal
Search more