Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 87 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of human brain (cerebellum) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.