Western blot analysis of extracts from 293 (human), NIH/3T3 (mouse), and C6 (rat) cells, untreated or UV-stimulated, using Phospho-Stat1 (Ser727) Antibody
Confocal immunofluorescent analysis of HeLa cells, untreated (left), IFN-γ treated (center) or IFN-γ and phosphatase treated (right), labeled with Phospho-Stat1 (Ser727) Antibody (green) and Pan-Keratin (C11) Mouse mAb #4545 (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either 10 μl of Phospho-Stat1 (Ser727) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP ® Human TAP1 Promoter Primers #5148, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.