Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-60438 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
Immunohistochemistry: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- PolE3/p17 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PolE3/p17 1:100. Detection: DAB staining using Immunohistochemistry Accessory Kit