Western Blot: CBX6 Antibody [NBP1-28731] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CBX6 antibody NBP1-28731 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. CBX6 was also immunoprecipitated by rabbit anti-CBX6 antibody NBP1-28686, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunohistochemistry: CBX6 Antibody [NBP1-28731] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBX6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry: CBX6 Antibody [NBP1-28731] - FFPE section of human prostate carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.