Phospho-Stat3 (Ser727) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 9134
• Prices: $109.00, $287.00, $678.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IP ChIP
• Species Reactivities: Human, Mouse, Rat, Hamster, Bovine, Dog, Pig, Horse
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser727 of mouse Stat3
• Description: Rabbit Polyclonal
• Gene: STAT3

Product images

Western blot analysis of extracts from A172 cells, untreated (-) or UV-treated (100 mJ, 30 min; +) with or without λ phosphatase (+), using Phospho-Stat3 (Ser727) Antibody (upper) and Stat3 (79D7) Rabbit mAb #4904 (lower).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml, 30 min), and either 10 μl of Phospho-Stat3 (Ser727) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP ® Human c-Fos Promoter Primers #4663, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.