Phospho-Stat3 (Tyr705) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 9131
• Prices: $287.00, $678.00
• Sizes: 100 µl (10 western blots), 300 µl (30 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IP ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Chicken, Bovine, Dog
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3
• Description: Rabbit Polyclonal
• Gene: STAT3

Product images

Western blot analysis of extracts from CNTF-treated SK-N-MC cells and EGF-treated A431 cells, using Phospho-Stat3 (Tyr705) Antibody (upper) or Stat3 Antibody #9132 (lower).

Immunoprecipitation of extracts from CNTF-treated 35S-labeled SK-N-MC cells, using Phospho-Stat3 (Tyr705) Antibody (upper) or Stat3 Antibody #9132 (lower).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either 10 μl of Phospho-Stat3 (Tyr705) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP ® Human c-Fos Promoter Primers #4663, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.