Western Blot: Tau Antibody [NBP2-25163] - Stripe blot of crude rat brain extract. Tau protein is expressed as up to 9 different isoforms of different molecular weight and so appears as multiple closely spaced bands covering the region of the blot from 48kDa to 67kDa, with an additional band at 100kDa.
Immunocytochemistry/Immunofluorescence: Tau Antibody [NBP2-25163] - E18 primary rat hippocampal neurons (from Neuromics) were fixed and immunostained with NBP2-25163 (red channel). The cells were also stained in green with NB110-58869, the monoclonal antibody to UCHL1, an abundant cytoplasmic protein of neurons which is concentrated in the perikarya. Since the perikarya contain both UCHL1 and Tau, the red and green signals superimpose, giving a yellow color, while the processes, which contain relatively much more Tau, appear red. Blue stain is DAPI and reveals cell nuclei of some non neuronal cells in these cultures.