Western blot analysis of extracts from various cell lines using STF-1 (D1Z2A) XP ® Rabbit mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing DDK-tagged full-length human STF-1 (hSTF-1-DDK; +) or Myc/DDK-tagged full-length human LRH-1, isoform 2 (hLRH-1-Myc/DDK; +), using STF1 (D1Z2A) XP ® Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).
Immunoprecipitation of STF-1 from NCI-H295R cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or STF-1 (D1Z2A) XP ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using STF-1 (D1Z2A) XP ® Rabbit mAb.
Confocal immunofluorescent analysis of NCI-H295R (positive, left) and SW-13 (negative, right) cells using STF-1 (D1Z2A) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 NCI-H295R cells treated with dibutyryl cAMP (0.4mM) for 1h and either 10 µl of STF-1 (D1Z2A) XP ® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CYP17A1 intron 1 primers, SimpleChIP ® Human StAR Intron 1 Primers #12864, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.