Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs) untreated (-) or treated (+) with 2 μg/mL LPS for 24 hours and THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 10 μg/mL LPS for 3 hours and 200 nM PMA for 24 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human IL-37/IL-1F7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1975) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-37/IL-1F7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.