Western blot analysis of extracts from HeLa cells, untreated (-) or UV-treated (2 hr; +), using Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) MultiMab™ Rabbit mAb mix. Western blot was imaged using Odyssey ® Infrared Imaging System (LI-COR ® Biotechnologies).
Immunoprecipitation of HeLa cells, untreated (-) or UV-treated (2 hr; +) (lanes 3 and 4), using Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) MultiMab™ Rabbit mAb mix. Lanes 1 and 2 are 10% input. Western blot analysis was performed using Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) MultiMab™ Rabbit mAb mix (upper) and Chk1 (2G1D5) Mouse mAb #2360 (lower).